Proven antibodies for HUVEC

This table summarizes useful antibodies for endothelial cell research that we have found work well in HUVEC.

Target Company Cat. # Host WB IP IF
β-catenin BD Transduction Laboratories 610153 mouse
Y
Y
caveolin 1 Abcam ab2910 rabbit
Y
CD63 Serotec C40320 mouse
Y*
Cdc42 BD Transduction Laboratories 610928 mouse
Y
Y
EEA1 BD Transduction Laboratories 610465 mouse
Y
Y
ERK1/2 Cell Signaling Technology 9102 rabbit
Y
phospho-ERK1/2 Cell Signaling Technology 9106 mouse
Y
Phospho-ERM Cell Signaling Technology 3141 rabbit
Y
Y
ICAM1 Abcam ab2213 mouse
Y
β1-integrin BD Transduction Laboratories 610468 mouse
Y
β3-integrin Cell Signaling Technology 4702 rabbit
Y
Nrp1 Santa Cruz sc7239 goat
Y
PECAM-1 R&D Systems BBA7
Y
podocalyxin Santa Cruz sc23903
Y
Y
Rab5 BD Transduction Laboratories 610281
Y
Y
RhoJ Abcam ab57584
Y
N
α-tubulin Sigma Aldrich T5618 mouse
Y
Y
γ-tubulin Sigma Aldrich T6557 mouse
Y
VE-cadherin BD Biosciences 555661 mouse
Y
Y
VEGFR1 Abcam 32152 rabbit
Y
VEGFR2 Cell Signaling Technology 2479 rabbit
Y
Y
VEGFR2 Abcam ab11939 rabbit
Y
VEGFR2 R&D MAB3573 mouse
Y
Y
phospho-VEGFR2 Cell Signaling Technology 2478 rabbit
Y

*needs saponin permeabilization protocol for IF

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